cd61 integrin β3  (Miltenyi Biotec)

Journal: Advanced Science

Article Title: Hybrid Membrane‐Coated Nanoparticles for Precise Targeting and Synergistic Therapy in Alzheimer's Disease

doi: 10.1002/advs.202306675

Figure Lengend Snippet: Extraction and hybridization of platelet membranes and CCR2‐RFP HEK293T cell membranes. A) Marker proteins (CD41, CD61, and CD62P) located in the membrane in different cell components of platelets. WC: whole cell; CM: cell membrane. B) Marker proteins (CCR2‐RFP and Na‐K‐ATPase) located in the membrane in different cell components of HEK293T cells. WC: whole cell; CM: cell membrane; SN: supernatant. C) Expression of marker proteins in the single cell membrane and hybrid cell membrane. D) Colocalization analysis of the hybrid cell membrane using Pearson's R 2 ( n = 3, *** p < 0.001). E) Fluorescence images of colocalization in hybrid cell membranes with different fusion ratio (red: CCR2‐RFP HEK293T cell membrane; green: platelet cell membrane; scale bar: 200 µm).

Article Snippet: The following primary antibodies were used: RFP antibody (1:1000, ChromoTek, Germany), α1‐sodium potassium ATPase antibody (1:1000, Abcam, USA), MCP1 antibody (1:1000, Abcam, USA), integrin β3 (CD61) antibody (1:1000, CST, USA), CD41 antibody (1:1000, Abcam, USA), CD62P antibody (1:1000, ABclonal, China), APP antibody (1:1000, CST, USA), p‐mTOR antibody (1:1000, CST, USA), mTOR antibody (1:1000, CST, USA), EPHX2 antibody (1:1000, ABclonal, China), LC3 II/I antibody (1:1000, CST, USA), Flag antibody (1:5000, ABclonal, China), GAPDH‐HRP antibody (1:10000, ABclonal, China) and β‐actin‐HRP antibody (1:10000, HuaBio, China).

Techniques: Extraction, Hybridization, Marker, Membrane, Expressing, Fluorescence